CORNUS MAS DEER SKIN

Moreover, myriocin up-regulated microphthalmia-associated transcription factor (MITF) and tyrosinase expression via phosphorylation of CREB, but it did not directly activate tyrosinase, a rate-limiting melanogenic enzyme. It was found that myriocin increased melanin synthesis in a concentration-dependent manner. The purpose of this study was to investigate effects of myriocin, an inhibitor of serine palmitoyltransferase, on melanogenesis. Li, Hailan Yun, Hye-Young Baek, Kwang Jin Kwon, Nyoun Soo Park, Kyoung-Chan Kim, Dong-Seok

CORNUS MAS DEER SKIN

Myriocin, a serine palmitoyltransferase inhibitor, increases melanin synthesis in Mel-Ab cells and a skin equivalent model. CCC+BMP7 BMSCs promote the ability of repairing ANFH in rats, providing a new therapeutic paradigm for the treatment of ANFH. Our results suggest that CCC+BMP7 significantly promote the proliferation and osteogenic differentiation of BMSCs in vitm. X-ray and microscopic observations revealed that ANFH was significantly improved and femoral head cells gradually recovered in rats treated with CCC+BMP7 BMSCs. In rats with induced ANFH, general observation scores of the CCC+BMP7 injected group were significantly higher than the model group. For in vivo assays, ANFH was induced in rats, and BMSCs were injected into the femoral head of the lower left extremity. The number of calcified nodules in the CCC+BMP7 group was significantly higher than that in other groups. The osteogenesis related genes, COLI, and integrin-Î☒, -Î±5, and -Î☡, were expressed significantly higher in CCC+BMP7 BMSCs. ALP activity and osteaocalcin content were significantly increased in CCC+BMP7 BMSCs. CCC+BMP7 BMSCs showed higher proliferation rate. Cell proliferation, alkaline phosphatase (ALP) activity, osteogenesis related gene expression, osteocalcin levels, and calcified nodules were quantified and compared between four groups: untreated controls, BMSCs cultured with CCC complex medium, BMP7-overexpressing BMSCs, and BMP7-overexpressing BMSCs cultured with CCC complex medium (CCC+BMP7). BMP7-overexpressing BMSCs were generated by lentiviral-mediated gene transduction. In the present study, we investigated the combined effect of Colla Comus Cervi (CCC) and BMP7-overexpressing bone marrow-derived mesenchymal stem cells (BMSCs) on osteogenic induction and the treatment of avascular necrosis of the femoral head (ANFH). Wang, Ping Shi, Bin Gao, Zhi-Hui Sun, Tie-Feng Yang, Wu-Bin Han, Shu-Fang Liu, Peng Wang, Lei-Lei Zhao, Bo-Nian Wang, Dan-Dan Â© 2013 Society of Cosmetic Scientists and the SociÃ©tÃ© FranÃ§aise de CosmÃ©tologie.ĮFFECT OF COLLA CORNUS CERVI COMBINED WITH LV-MEDIATED BMP7 TRANSFECTED BMSCs ON ANFH IN RATS. Therefore, our results showed that combined use of HA and CCC can be helpful for the reconstruction of SE with good stem cell activity and epidermal differentiation.

To avoid these limitations, SEs can be used for testing the safety or the efficacy of cosmetic ingredients. Legislation in the EU prohibits marketing cosmetics and personal care products that contain constituents that have been examined through animal experiments. In conclusion, simultaneous presence of HA and CCC contributed to the stem cell activity and epidermal differentiation in the reconstruction of SE. It showed that epidermal differentiation was significantly improved by addition of CCC. Furthermore, filaggrin expression was much increased when CCC was added. These finding showed that addition of HA and/or CCC may affect the stem cell activity in the reconstruction of skin. Increased staining of integrin Î☖ and Î☡ was variably observed when HA and/or CCC was added to make dermal substitute. But, immunohistochemical staining showed that p63 was significantly increased by addition of HA and/or CCC. Results showed similar morphologic findings when hyaluronic acid (HA) and/or CCC was added. Four different models were constructed according to different composition of dermal substitute. The aim of this study was to investigate the effects of Cervi cornus Colla (CCC) on the stem cell activity and epidermal differentiation in the reconstruction of skin equivalent. In the reconstruction of skin equivalents (SEs), keratinocyte differentiation is important because epidermal differentiation is closely related with barrier function.

Cervi cornus Colla (deer antler glue) induce epidermal differentiation in the reconstruction of skin equivalents.Ĭhoi, H-R Nam, K-M Kim, D-S Huh, C-H Na, J-I Park, K-C